Screening of Factor V G1691A (Leiden) and Factor II/prothrombin G20210A Polymorphisms among Apparently Healthy Taif-Saudi Arabia Population Using a Reverse Hybridization Strip Assay Approach

Single nucleotide polymorphisms (SNPs) Factor V G1691A and Factor II G20210A have been shown to play a role in both venous and arterial thrombosis. Several studies have been carried out to estimate the prevalence of Factor V G1691A and the Factor II/prothrombin G20210A gene polymorphisms among healthy subjects. The aim of the present work is to study the prevalence and allele frequency of factor V G1691A (Leiden) and factor II/prothrombin G20210A polymorphisms among healthy Taif-Saudi Arabia population. The SNPs genotyping was carried out via Cardiovascular Disease (CVD) Strip Assay which is based on a Polymerase Chain Reaction-Reverse hybridization technique and DNA from 200 unrelated Saudi healthy subjects. The mutant (AA) genotype of both studied polymorphisms was completely absent. The prevalence of FV-Leiden and factor II/prothrombin G20210A were 2% and 1.5% respectively. This study identify the prevalence of Factor V G1691A and the Factor II/prothrombin G20210A polymorphisms which were generally comparable to the rates established for countries of Caucasian descent.